Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZNF75 / ZNF75D Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114842-100
Catalog Number: LS-G114842
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF75(ZNF75D) (NM_007131), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- D8C6 | ZNF75 | ZNF82 | Zinc finger protein 75 | Zinc finger protein 82 | ZNF75D | Zinc finger protein 75D | ZKSCAN24 | ZSCAN28 | Zinc finger protein 75 (D8C6)
- Origin Species
- Human
- NCBI GenBank
- S67970
- SwissProt
- P51815
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAMRELNADS CSSPQMGAMW ETSGSVKENS SQSKKYSTKI ENLGPESACR HFWSFRYHEA61TGPLETISQL QKLCHQWLRP EIHSKEQILE MLVLEQFLSI LPKETQNWVQ KHHPQNVKQA121LVLVEFLQRE PDGTKNEVTA HELGKEAVLL GGTAVAPGFK WKPAEPQPMG VFQKEYWNTY181RVLQEQLGWN THKETQPVYE RAVHDQQMLA LSEQKRIKHW KMASKLILPE SLSLLTFEDV241AVYFSEEEWQ LLNPLEKTLY NDVMQDIYET VISLGLKLKN DTGNDHPISV STSEIQTSGC301EVSKKTRMKI AQKTMGRENP GDTHSVQKWH RAFPRKKRKK PATCKQELPK LMDLHGKGPT361GEKPFKCQEC GKSFRVSSDL IKHHRIHTGE KPYKCQQCDR RFRWSSDLNK HFMTHQGIKP421YRCSWCGKSF SHNTNLHTHQ RIHTGEKPFK CDECGKRFIQ NSHLIKHQRT HTGEQPYTCS481LCKRNFSRRS SLLRHQKLHR RREACLVSPNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
510 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.