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ZNF749 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF749 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G121144-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF749 (NM_001023561), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 749 | ZNF749
Origin Species
Human
NCBI GenBank
NM_001023561
SwissProt
O43361

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
90.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNLTEDCMVF EDVAIYFSQE EWGILNDAQR HLHSNVMLEN FALLSSVGCW HGAKDEEVPS
61KQCVSVRVLQ VTIPKPALST LKAQPCKMCS SILKDILHLA EHDGTHPEQG LYTCAAEHDL
121HQKEQIREKL TRSDEWRPSF VNHSAHVGER NFTCTQGGKD FTASSDLLQQ QVLNSGWKLY
181RDTQDGEAFQ GEQNDFNSSQ GGKDFCHQHG LFEHQKTHNG ERPYEFSECG ELFRYNSNLI
241KYQQNHAGER PYEGTEYGKT FIRKSNLVQH QKIHSEGFLS KRSDPIEHQE ILSRPTPYEC
301TQCGKAFLTQ AHLVGHQKTH TGEQPYECNK CGKFFMYNSK LIRHQKVHTG ERRYECSECG
361KLFMDSFTLG RHQRVHTGER PFECSICGKF FSHRSTLNMH QRVHAGKRLY KCSECGKAFS
421LKHNVVQHLK IHTGERPYEC TECEKAFVRK SHLVQHQKIH TDAFSKRSDL IQHKRIDIRP
481RPYTCSECGK AFLTQAHLVG HQKIHTGERP YECTQCAKAF VRKSHLVQHE KIHTDAFSKR
541SDLIQHKRID LRPRPYVCSE CGKAFLTQAH LDGHQKIQTG ERRYECNECG KFFLDSYKLV
601IHQRIHTGEK PYKCSKCGKF FRYRCTLSRH QKVHTGERPY ECSECGKFFR DSYKLIIHQR
661VHTGEKPYEC SNCGKFLRYR STFIKHHKVC TGEKPHECSK CRELFRTKSS LIIHQQSHTG
721ESPFKLRECG KDFNKCNTGQ RQKTHTGERS YECGESSKVF KYNSSLIKHQ IIHTGKRP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

778 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.