LSBio
ZNF713 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF713 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G104170-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF713 (NM_182633), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 713 | ZNF713
Origin Species
Human
NCBI GenBank
NM_182633
SwissProt
Q8N859

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
50 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEEEEMNDGS QMVRSQESLT FQDVAVDFTR EEWDQLYPAQ KNLYRDVMLE NYRNLVALGY
61QLCKPEVIAQ LELEEEWVIE RDSLLDTHPD GENRPEIKKS TTSQNISDEN QTHEMIMERL
121AGDSFWYSIL GGLWDFDYHP EFNQENHKRY LGQVTLTHKK ITQERSLECN KFAENCNLNS
181NLMQQRIPSI KIPLNSDTQG NSIKHNSDLI YYQGNYVRET PYEYSECGKI FNQHILLTDH
241IHTAEKPSEC GKAFSHTSSL SQPQMLLTGE KPYKCDECGK RFSQRIHLIQ HQRIHTGEKP
301FICNGCGKAF RQHSSFTQHL RIHTGEKPYK CNQCGKAFSR ITSLTEHHRL HTGEKPYECG
361FCGKAFSQRT HLNQHERTHT GEKPYKCNEC GKAFSQSAHL NQHRKIHTRE KLCEYKCEQT
421VRHSPSFSST
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

430 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.