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ZNF710 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF710 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G103634-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF710 (NM_198526), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 710 | ZNF710
Origin Species
Human
NCBI GenBank
AA417675
SwissProt
Q8N1W2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEGFMDSGTQ TDAVVVLSLA QAAVLGLVSE NELFGATISA EAFYPDLGPE LSGAAMGEPE
61PPGPDVYQLA CNGRALEEPA EEEVLEVEAA CEKHTRRKTR PPVRLVPKVK FEKVEEEEQE
121VYEVSVPGDD KDAGPAEAPA EAASGGCDAL VQSSAVKMID LSAFSRKPRT LRHLPRTPRP
181ELNVAPYDPH FPAPARDGFP EPSMALPGPE ALPTECGFEP PHLAPLSDPE APSMESPEPV
241KPEQGFVWQE ASEFEADTAG STVERHKKAQ LDRLDINVQI DDSYLVEAGD RQKRWQCRMC
301EKSYTSKYNL VTHILGHNGI KPHSCPHCSK LFKQPSHLQT HLLTHQGTRP HKCQVCHKAF
361TQTSHLKRHM LLHSEVKPYS CHFCGRGFAY PSELKAHEVK HESGRCHVCV ECGLDFSTLT
421QLKRHLASHQ GPTLYQCLEC DKSFHYRSQL QNHMLKHQNV RPFVCTECGM EFSQIHHLKQ
481HSLTHKGVKE FKCEVCGREF TLQANMKRHM LIHTSVRPYQ CHICFKTFVQ KQTLKTHMIV
541HSPVKPFKCK VCGKSFNRMY NLLGHMHLHA GSKPFKCPYC SSKFNLKGNL SRHMKVKHGV
601MDIGLDSQDP MMELTGTDPS ELDGQQEMED FEENAYSYAS VDSSAEASVL TEQAMKEMAY
661YNVL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

664 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.