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ZNF703 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF703 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G109628-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF703 (NM_025069), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZEPPO1 | ZNF703 | ZPO1 | ZNF503L
Origin Species
Human
NCBI GenBank
NM_025069
SwissProt
Q9H7S9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSDSPAGSNP RTPESSGSGS GGGGKRPAVP AAVSLLPPAD PLRQANRLPI RVLKMLSAHT
61GHLLHPEYLQ PLSSTPVSPI ELDAKKSPLA LLAQTCSQIG KPDPPPSSKL NSVAAAANGL
121GAEKDPGRSA PGAASAAAAL KQLGDSPAED KSSFKPYSKG SGGGDSRKDS GSSSVSSTSS
181SSSSSPGDKA GFRVPSAACP PFPPHGAPVS ASSSSSSPGG SRGGSPHHSD CKNGGGVGGG
241ELDKKDQEPK PSPEPAAVSR GGGGEPGAHG GAESGASGRK SEPPSALVGA GHVAPVSPYK
301PGHSVFPLPP SSIGYHGSIV GAYAGYPSQF VPGLDPSKSG LVGGQLSGGL GLPPGKPPSS
361SPLTGASPPS FLQGLCRDPY CLGGYHGASH LGGSSCSTCS AHDPAGPSLK AGGYPLVYPG
421HPLQPAALSS SAAQAALPGH PLYTYGFMLQ NEPLPHSCNW VAASGPCDKR FATSEELLSH
481LRTHTALPGA EKLLAAYPGA SGLGSAAAAA AAAASCHLHL PPPAAPGSPG SLSLRNPHTL
541GLSRYHPYGK SHLSTAGGLA VPSLPTAGPY YSPYALYGQR LASASALGYQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

590 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.