LSBio
ZNF701 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF701 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111875-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF701 (NM_018260), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZNF701 | Zinc finger protein 701
Origin Species
Human
NCBI GenBank
NM_018260

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.7 kda
Protein Type
Lysate
Amino Acid Sequence
1MALPQGLLTF RDVAIEFSQE EWKCLDPAQR TLYRDVMLEN YRNLVSLDTS SKCMMKMFSS
61TGQGNTEVVH TGTLQIHASH HIGDTCFQEI EKDIHDFVFQ WQENETNGHE ALMTKIKKLM
121SSTERHDQRH AGNKPIKNEL GSSFHSHLPE VHIFHPEGKI GNQVEKAIND AFSVSASQRI
181SCRPKTRISN KYRNNFLQSS LLTQKREVHT REKSFQRNES GKAFNGSSLL KKHQIIHLGD
241KQYKCDVCGK DFHQKRYLAC HRCHTGENPY KCNECGKTFS HNSALLVHKA IHTGEKPYKC
301NECGKVFNQQ SNLARHHRVH TGEKPYKCEE CDKVFSRKSH LERHRRIHTG EKPYKCKVCD
361KAFRRDSHLA QHTVIHTGEK PYKCNECGKT FVQNSSLVMH KVIHTGEKRY KCNECGKVFN
421HKSNLACHRR LHTGEKPYKC NECGKVFNRK SNLERHHRLH TGKKS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

465 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.