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ZNF687 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF687 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF687 antibodies →
$504.00each
Catalog Number: LS-G110942-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF687 (NM_020832), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZNF687 | Zinc finger protein 687 | KIAA1441 | RP11-126K1.3
Origin Species
Human
NCBI GenBank
AI498161
SwissProt
Q8N1G0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
129.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGDMKTPDFD DLLAAFDIPD IDANEAIHSG PEENEGPGGP GKPEPGVGSE SEDTAAASAG
61DGPGVPAQAS DHGLPPPDIS VVSVIVKNTV CPEQSEALAG GSAGDGAQAA GVTKEGPVGP
121HRMQNGFGSP EPSLPGTPHS PAPPSGGTWK EKGMEGKTPL DLFAHFGPEP GDHSDPLPPS
181APSPTREGAL TPPPFPSSFE LAQENGPGMQ PPVSSPPLGA LKQESCSPHH PQVLAQQGSG
241SSPKATDIPA SASPPPVAGV PFFKQSPGHQ SPLASPKVPV CQPLKEEDDD EGPVDKSSPG
301SPQSPSSGAE AADEDSNDSP ASSSSRPLKV RIKTIKTSCG NITRTVTQVP SDPDPPAPLA
361EGAFLAEASL LKLSPATPTS EGPKVVSVQL GDGTRLKGTV LPVATIQNAS TAMLMAASVA
421RKAVVLPGGT ATSPKMIAKN VLGLVPQALP KADGRAGLGT GGQKVNGASV VMVQPSKTAT
481GPSTGGGTVI SRTQSSLVEA FNKILNSKNL LPAYRPNLSP PAEAGLALPP TGYRCLECGD
541AFSLEKSLAR HYDRRSMRIE VTCNHCARRL VFFNKCSLLL HAREHKDKGL VMQCSHLVMR
601PVALDQMVGQ PDITPLLPVA VPPVSGPLAL PALGKGEGAI TSSAITTVAA EAPVLPLSTE
661PPAAPATSAY TCFRCLECKE QCRDKAGMAA HFQQLGPPAP GATSNVCPTC PMMLPNRCSF
721SAHQRMHKNR PPHVCPECGG NFLQANFQTH LREACLHVSR RVGYRCPSCS VVFGGVNSIK
781SHIQTSHCEV FHKCPICPMA FKSGPSAHAH LYSQHPSFQT QQAKLIYKCA MCDTVFTHKP
841LLSSHFDQHL LPQRVSVFKC PSCPLLFAQK RTMLEHLKNT HQSGRLEETA GKGAGGALLT
901PKTEPEELAV SQGGAAPATE ESSSSSEEEE VPSSPEPPRP AKRPRRELGS KGLKGGGGGP
961GGWTCGLCHS WFPERDEYVA HMKKEHGKSV KKFPCRLCER SFCSAPSLRR HVRVNHEGIK
1021RVYPCRYCTE GKRTFSSRLI LEKHVQVRHG LQLGAQSPGR GTTLARGSSA RAQGPGRKRR
1081QSSDSCSEEP DSTTPPAKSP RGGPGSGGHG PLRYRSSSST EQSLMMGLRV EDGAQQCLDC
1141GLCFASPGSL SRHRFISHKK RRGVGKASAL GLGDGEEEAP PSRSDPDGGD SPLPASGGPL
1201TCKVCGKSCD SPLNLKTHFR THGMAFIRAR QGAVGDN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1237 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.