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ZNF682 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF682 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G108372-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Zinc finger protein 682(ZNF682) (NM_033196), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BC39498_3 | ZNF682 | Zinc finger protein 682
Origin Species
Human
NCBI GenBank
NM_033196
SwissProt
O95780

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MELLTFRDVT IEFSLEEWEF LNPAQQSLYR KVMLENYRNL VSLGLTVSKP ELISRLEQRQ
61EPWNVKRHET IAKPPAMSSH YTEDLLPEQC MQDSFQKVIL RRYGSCGLED LHLRKDGENV
121GECKDQKEIY NGLNQCLSTL PSKIFPYNKC VKVFSKSSNL NRENIRHTTE KLFKCMQCGK
181VFKSHSGLSY HKIIHTEEKL CICEECGKTF KWFSYLTKHK RIHTGEKPYK CEECGKAFNW
241CSSLTKHKRI HTGEKPYKCE ECGKAFHWCS PFVRHKKIHT GEKPYTCEDC GRAFNRHSHL
301TKHKTIHTGK KPYKCKECGK AFNHCSLLTI HERTHTGEKP YKCEECGKAF NSSSILTEHK
361VIHSGEKPYK CEKCDKVFKR FSYLTKHKRI HTGEKPYKCE ECGKAFNWSS ILTEHKRIHT
421GEKPYNCEEC GKAFNRCSHL TRHKKIHTAV KRYKCEECGK AFKRCSHLNE HKRVQRGEKS
481CKYKKCGEAF NHCSNLTT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

498 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.