LSBio
ZNF677 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF677 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G104220-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF677 (NM_182609), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Hypothetical protein MGC48625 | Zinc finger protein 677 | ZNF677
Origin Species
Human
NCBI GenBank
AK026366
SwissProt
Q86XU0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MALSQGLFTF KDVAIEFSQE EWECLDPAQR ALYRDVMLEN YRNLLSLDED NIPPEDDISV
61GFTSKGLSPK ENNKEELYHL VILERKESHG INNFDLKEVW ENMPKFDSLW DYDVKNYKGM
121PLTCNKNLTH RKDQQHNKSS IHFSLKQSVS IRDSAHQYFI HDKPFIRNLL KLKNNIRYAG
181NKYVKCFENK IGLSLQAQLA ELQRFQTGEK MYECNPVEKS INSSSVSPLP PCVKNICNKY
241RKILKYPLLH TQYGRTHIRE KSYKCNDCGK AFSKSSNLTN HQRIHSGQRP YKCNECGKAF
301NQCSNLTRHQ RVHTGEKPYQ CNICGKVCSQ NSNLASHQRM HTGEKPYKCN ECGKAFIQRS
361HLWGHERIHT GEKPYKCNEC DKAFAERSSL TQHKRIHTGE KPYICNECGK AFKQCSHLTR
421HQNIHPGEKP HKCNVCGRAF IQSSSLVEHQ RIHTGEKPYK CNKCDKAFIK RSHLWGHQRT
481HTGEKPYKCT ECGKAFTERS NLTQHKKIHT GEKPYKCTEC GKAFTQFANL TRHQKIHIEK
541KHCKHNIHGN ALFQSSNLGD HQKSYNREKH IKYNETKIKY SSCT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

584 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.