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ZNF668 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF668 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G109852-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Zinc finger protein 668(ZNF668) (NM_024706), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 668 | ZNF668
Origin Species
Human
NCBI GenBank
NM_024706

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEVEAAEARS PAPGYKRSGR RYKCLSCTKT FPNAPRAARH AATHGPADCS EEVAEVKPKP
61ETEAKAEEAS GEKVSGSAAK PRPYACPLCP KAYKTAPELR SHGRSHTGEK PFPCPECGRR
121FMQPVCLRVH LASHAGELPF RCAHCPKAYG ALSKLKIHQR GHTGERPYAC ADCGKSFADP
181SVFRKHRRTH AGLRPYSCER CGKAYAELKD LRNHERSHTG ERPFLCSECG KSFSRSSSLT
241CHQRIHAAQK PYRCPACGKG FTQLSSYQSH ERTHSGEKPF LCPRCGRMFS DPSSFRRHQR
301AHEEVKPYHC EKCGKDFRQP ADLAMHRRVH TGDRPFKCLQ CDKTFVASWD LKRHALVHSG
361QRPFRCEECG RAFAERASLT KHSRVHSGER PFHCNACGKS FVVSSSLRKH ERTHRSSEAA
421GVPPAQELVV GLALPVGVAG ESSAAPAAGA GLGDPPAGLL GLPPESGGVM ATQWQVVGMT
481VEHVECQDAG VREAPGPLEG AGEAGGEEAD EKPPQFVCRE CKETFSTMTL LRRHERSHPE
541LRPFPCTQCG KSFSDRAGLR KHSRTHSSVR PYTCPHCPKA FLSASDLRKH ERTHPVPMGT
601PTPLEPLVAL LGMPEEGPA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

619 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.