Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZNF667 / MIPU1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G110464-100
Catalog Number: LS-G110464
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF667 (NM_022103), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MIPU1 | Zinc finger protein 667 | ZNF667
- Origin Species
- Human
- NCBI GenBank
- NM_022103
- SwissProt
- Q5HYK9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 70 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MPSARGKSKS KAPITFGDLA IYFSQEEWEW LSPIQKDLYE DVMLENYRNL VSLGLSFRRP61NVITLLEKGK APWMVEPVRR RRAPDSGSKC ETKKLPPNQC NKSGQSICQK LVSAQQKAPT121RKSGCNKNSV LVKPKKGHSG KKPLKCNDCG KTFSRSFSLK LHQNIHTGEK PFECSNCRKA181FRQISSILLH QRIHSGKKSH ECNKCGESFN QRTTLILHMR IHDGKEILDC GKALSQCQSF241NIHQKIHVVG NVCQCRKCGR AFNQMSSLLL HKKIHNGKKT HKYNKCGRGF KKKSVFVVHK301RIHAGEKIPE NAKALSQSLQ QRSHHLENPF KCRKCGKLFN RISPLMLHQR IHTSEKPYKC361DKCDKFFRRL STLILHLRIH NGEKLYRCNK CEKVCNRHSS LIQHQKVHTK KKKLFECKEC421GKMFSGTANL KIHQNIHSEE KPFKCNKCSK VFGRQSFLIE HQRIHTGEKP YQCEECGKAF481SHRISLTRHK RIHTEDRPYE CDQCGKAFSQ SAHLAQHERI HTGEKPYTCK TCGKAFSQRT541SLILHERSHT GEKPYECNEC GKAFSSGSDL IRHQRSHSSE KPYECSKCGK AYSRSSSLIR601HQNTHSEEKATRTRPLEQKLISEEDLAANDILDYKDDDDKV
610 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.