Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZNF659 / ZNF385D Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109843-100
Catalog Number: LS-G109843
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF385D (NM_024697), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ZNF659 | Zinc finger protein 385D | Zinc finger protein 659 | ZNF385D
- Origin Species
- Human
- NCBI GenBank
- NM_024697
- SwissProt
- Q9H6B1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 42.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MRNIMYFGGT CQSPALPALV RPPAPPLQPS LDIKPFLPFP LDTAAAVNLF PNFNAMDPIQ61KAVINHTFGV PLPHRRKQII SCNICQLRFN SDSQAAAHYK GTKHAKKLKA LEAMKNKQKS121VTAKDSAKTT FTSITTNTIN TSSDKTDGTA GTPAISTTTT VEIRKSSVMT TEITSKVEKS181PTTATGNSSC PSTETEEEKA KRLLYCSLCK VAVNSASQLE AHNSGTKHKT MLEARNGSGT241IKAFPRAGVK GKGPVNKGNT GLQNKTFHCE ICDVHVNSET QLKQHISSRR HKDRAAGKPP301KPKYSPYNKL QKTAHPLGVK LVFSKEPSKP LAPRILPNPL AAAAAAAAVA VSSPFSLRTA361PAATLFQTSA LPPALLRPAP GPIRTAHTPV LFAPYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
395 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.