LSBio
ZNF654 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF654 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G111818-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF654 (NM_018293), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Melanoma-associated antigen | Zinc finger protein 654 | ZNF654
Origin Species
Human
NCBI GenBank
NM_018293
SwissProt
Q8IZM8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLCNKEFLGG HIVRHAQAHQ KKGSFACVIC GRKFRNRGLM QKHLKNHVKK IQRQQIAAAQ
61QDDQEVTALE EINCSSSSIS FENGNSDSKD LEVETLTASS EGNKEVIPEH VAEFIEIPIS
121VPEDVIENVI ENGSPNNSLN NVFKPLTECG DDYEEEEDEE GDYEEDDYDL NQETSVIHKI
181NGTVCHPKDI YATDQEGNFK CPALGCVRIF KRIGFLNKHA MTVHPTDLNV RQTVMKWSKG
241KCKFCQRQFE DSQHFIDHLN RHSYPNVYFC LHFNCNESFK LPFQLAQHTK SHRTFQAQCS
301FPECHELFED LPLLYEHEAQ HYLSKTPESS AQPSETILWD VQTDSNPNQE KDSSSNEKQT
361ISLPVSTSKS RKESTEPKTC IESMEKKTDS LVQNGNERSD DTVSNISLID QKMPDIEPNS
421ENNCSSSDIV NGHSEIEQTP LVSSDPALKI DTNRIRTENG SILPSVVPQE HNTLPVSQAP
481SKPNLTSEHT SYGLILTKPY VRPLPPSYLD ERYLSMPKRR KFLTDRVDAC SDQDNVYKKS
541VKRLRCGKCL TTYCNAEALE AHLAQKKCQT LFGFDSDDES A
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

581 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.