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ZNF652 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF652 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G113615-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF652 (NM_014897), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0924 | Zinc finger protein 652 | ZNF652
Origin Species
Human
NCBI GenBank
AB023141
SwissProt
Q9Y2D9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSHTASSCQE LVENCAVHVA GMAQEDSRRG QVPSSFYHGA NQELDLSTKV YKRESGSPYS
61VLVDTKMSKP HLHETEEQPY FRETRAVSDV HAVKEDRENS DDTEEEEEEV SYKREQIIVE
121VNLNNQTLNV SKGEKGVSSQ SKETPVLKTS SEEEEEESEE EATDDSNDYG ENEKQKKKEK
181IVEKVSVTQR RTRRAASVAA ATTSPTPRTT RGRRKSVEPP KRKKRATKEP KAPVQKAKCE
241EKETLTCEKC PRVFNTRWYL EKHMNVTHRR MQICDKCGKK FVLESELSLH QQTDCEKNIQ
301CVSCNKSFKK LWSLHEHIKI VHGYAEKKFS CEICEKKFYT MAHVRKHMVA HTKDMPFTCE
361TCGKSFKRSM SLKVHSLQHS GEKPFRCENC DERFQYKYQL RSHMSIHIGH KQFMCQWCGK
421DFNMKQYFDE HMKTHTGEKP FICEICGKSF TSRPNMKRHR RTHTGEKPYP CDVCGQRFRF
481SNMLKAHKEK CFRVTSPVNV PPAVQIPLTT SPATPVPSVV NTATTPTPPI NMNPVSTLPP
541RPIPHPFSHL HIHPHPHHPH HLPIPPVPHL PPPPALFKSE PLNHRGQSED NFLRHLAEKN
601SSAQHH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

606 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.