LSBio
ZNF648 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF648 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF648 antibodies →
$725.00each
Catalog Number: LS-G121679-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF648 (NM_001009992), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 648 | ZNF648
Origin Species
Human
SwissProt
Q5T619

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAQVDSQDRW GEASPLSSLT EEAHDTQMLS MNLESDDEDG GEAEKEGTAD PVACPRGSSP
61VTHENPDLPW PHPLGKEEEK FSDSSSAGGM GQKPVEMSGK ASWSRDVTKI NETQGSPGAS
121RALGSLPSGL AHKLLGQMQP LGDRLPAGDD GYSGANQDAV LDVPPSFPSN GKYLCAHKSV
181DTSAGNSSLL CFPRPGSNWD LPTQETHTPA QASATPASLA AAVLAKARNS RKVQNQAGRR
241EGGEAEARPY RCLRGGRAFQ KPSKPLSPAE TRGGAAKRYA CELCGKAYSH RGTLQQHRRL
301HTGERPYQCS FCDKAYTWSS DHRKHIRTHT GEKPYPCPDC GKAFVRSSDL RKHQRNMHSN
361NKPFPCSECG LTFNKPLSLL RHQRTHLGAK PFRCPACDRE FAVASRMVEH QRVHSGERPF
421PCPTCGKCFT KSSNLSEHQT LHTGQRPFKC ADCGVAFAQP SRLVRHQRIH TGERPFPCTQ
481CGQAFARSST LKRHQQIHSG EKGFLCAECG RAFRIASELA QHIRMHNGER PYQCEDCGQA
541FTRSNHLQRH RAKHGTCKKE PIPSSSDE
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

568 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.