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ZNF646 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF646 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G113743-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF646 (NM_014699), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0296 | ZNF646 | Zinc finger protein 646
Origin Species
Human
NCBI GenBank
NM_014699
SwissProt
O15015

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
200.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEDTPPSLSC SDCQRHFPSL PELSRHRELL HPSPNQDSEE ADSIPRPYRC QQCGRGYRHP
61GSLVNHRRTH ETGLFPCTTC GKDFSNPMAL KSHMRTHAPE GRRRHRPPRP KEATPHLQGE
121TVSTDSWGQR LGSSEGWENQ TKHTEETPDC ESVPDPRAAS GTWEDLPTRQ REGLASHPGP
181EDGADGWGPS TNSARAPPLP IPASSLLSNL EQYLAESVVN FTGGQEPTQS PPAEEERRYK
241CSQCGKTYKH AGSLTNHRQS HTLGIYPCAI CFKEFSNLMA LKNHSRLHAQ YRPYHCPHCP
301RVFRLPRELL EHQQSHEGER QEPRWEEKGM PTTNGHTDES SQDQLPSAQM LNGSAELSTS
361GELEDSGLEE YRPFRCGDCG RTYRHAGSLI NHRKSHQTGV YPCSLCSKQL FNAAALKNHV
421RAHHRPRQGV GENGQPSVPP APLLLAETTH KEEEDPTTTL DHRPYKCSEC GRAYRHRGSL
481VNHRHSHRTG EYQCSLCPRK YPNLMALRNH VRVHCKAARR SADIGAEGAP SHLKVELPPD
541PVEAEAAPHT DQDHVCKHEE EATDITPAAD KTAAHICSIC GLLFEDAESL ERHGLTHGAG
601EKENSRTETT MSPPRAFACR DCGKSYRHSG SLINHRQTHQ TGDFSCGACA KHFHTMAAMK
661NHLRRHSRRR SRRHRKRAGG ASGGREAKLL AAESWTRELE DNEGLESPQD PSGESPHGAE
721GNLESDGDCL QAESEGDKCG LERDETHFQG DKESGGTGEG LERKDASLLD NLDIPGEEGG
781GTHFCDSLTG VDEDQKPATG QPNSSSHSAN AVTGWQAGAA HTCSDCGHSF PHATGLLSHR
841PCHPPGIYQC SLCPKEFDSL PALRSHFQNH RPGEATSAQP FLCCLCGMIF PGRAGYRLHR
901RQAHSSSGMT EGSEEEGEEE GVAEAAPARS PPLQLSEAEL LNQLQREVEA LDSAGYGHIC
961GCCGQTYDDL GSLERHHQSQ SSGTTADKAP SPLGVAGDAM EMVVDSVLED IVNSVSGEGG
1021DAKSQEGAGT PLGDSLCIQG GESLLEAQPR PFRCNQCGKT YRHGGSLVNH RKIHQTGDFL
1081CPVCSRCYPN LAAYRNHLRN HPRCKGSEPQ VGPIPEAAGS SELQVGPIPE GGSNKPQHMA
1141EEGPGQAEVE KLQEELKVEP LEEVARVKEE VWEETTVKGE EIEPRLETAE KGCQTEASSE
1201RPFSCEVCGR SYKHAGSLIN HRQSHQTGHF GCQACSKGFS NLMSLKNHRR IHADPRRFRC
1261SECGKAFRLR KQLASHQRVH MERRGGGGTR KATREDRPFR CGQCGRTYRH AGSLLNHRRS
1321HETGQYSCPT CPKTYSNRMA LKDHQRLHSE NRRRRAGRSR RTAVRCALCG RSFPGRGSLE
1381RHLREHEETE REPANGQGGL DGTAASEANL TGSQGLETQL GGAEPVPHLE DGVPRPGERS
1441QSPIRAASSE APEPLSWGAG KAGGWPVGGG LGNHSGGWVP QFLTRSEEPE DSVHRSPCHA
1501GDCQLNGPTL SHMDSWDNRD NSSQLQPGSH SSCSQCGKTY CQSGSLLNHN TNKTDRHYCL
1561LCSKEFLNPV ATKSHSHNHI DAQTFACPDC GKAFESHQEL ASHLQAHARG HSQVPAQMEE
1621ARDPKAGTGE DQVVLPGQGK AQEAPSETPR GPGESVERAR GGQAVTSMAA EDKERPFRCT
1681QCGRSYRHAG SLLNHQKAHT TGLYPCSLCP KLLPNLLSLK NHSRTHTDPK RHCCSICGKA
1741FRTAARLEGH GRVHAPREGP FTCPHCPRHF RRRISFVQHQ QQHQEEWTVA GSGAPVAPVT
1801GRGDLPLPPP PTPTTPLLDP SPQWPADLSF SL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1832 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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