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ZNF639 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF639 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G112713-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF 639(ZNF639) (NM_016331), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ANC-2H01 | ANC_2H01 | ZASC1 | Zinc finger protein 639 | Zinc finger protein ANC_2H01 | ZNF639 | Zinc finger protein ZASC1
Origin Species
Human
NCBI GenBank
NM_016331
SwissProt
Q9UID6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
55.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNEYPKKRKR KTLHPSRYSD SSGISRIADG FNGIFSDHCY SVCSMRQPDL KYFDNKDDDS
61DTETSNDLPK FADGIKARNR NQNYLVPSPV LRILDHTAFS TEKSADIVIC DEECDSPESV
121NQQTQEESPI EVHTAEDVPI AVEVHAISED YDIETENNSS ESLQDQTDEE PPAKLCKILD
181KSQALNVTAQ QKWPLLRANS SGLYKCELCE FNSKYFSDLK QHMILKHKRT DSNVCRVCKE
241SFSTNMLLIE HAKLHEEDPY ICKYCDYKTV IFENLSQHIA DTHFSDHLYW CEQCDVQFSS
301SSELYLHFQE HSCDEQYLCQ FCEHETNDPE DLHSHVVNEH ACKLIELSDK YNNGEHGQYS
361LLSKITFDKC KNFFVCQVCG FRSRLHTNVN RHVAIEHTKI FPHVCDDCGK GFSSMLEYCK
421HLNSHLSEGI YLCQYCEYST GQIEDLKIHL DFKHSADLPH KCSDCLMRFG NERELISHLP
481VHETT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

485 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.