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ZNF629 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF629 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G120204-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF629 (NM_001080417), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DNA-binding protein | Zinc finger protein 629 | ZNF629 | ZNF65 | ZNF | Zinc finger protein 65 | KIAA0326
Origin Species
Human
NCBI GenBank
AB002324
SwissProt
Q9UEG4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
96.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEPETALWGP DLQGPEQSPN DAHRGAESEN EEESPRQESS GEEIIMGDPA QSPESKDSTE
61MSLERSSQDP SVPQNPPTPL GHSNPLDHQI PLDPPAPEVV PTPSDWTKAC EASWQWGALT
121TWNSPPVVPA NEPSLRELVQ GRPAGAEKPY ICNECGKSFS QWSKLLRHQR IHTGERPNTC
181SECGKSFTQS SHLVQHQRTH TGEKPYKCPD CGKCFSWSSN LVQHQRTHTG EKPYKCTECE
241KAFTQSTNLI KHQRSHTGEK PYKCGECRRA FYRSSDLIQH QATHTGEKPY KCPECGKRFG
301QNHNLLKHQK IHAGEKPYRC TECGKSFIQS SELTQHQRTH TGEKPYECLE CGKSFGHSST
361LIKHQRTHLR EDPFKCPVCG KTFTLSATLL RHQRTHTGER PYKCPECGKS FSVSSNLINH
421QRIHRGERPY ICADCGKSFI MSSTLIRHQR IHTGEKPYKC SDCGKSFIRS SHLIQHRRTH
481TGEKPYKCPE CGKSFSQSSN LITHVRTHMD ENLFVCSDCG KAFLEAHELE QHRVIHERGK
541TPARRAQGDS LLGLGDPSLL TPPPGAKPHK CLVCGKGFND EGIFMQHQRI HIGENPYKNA
601DGLIAHAAPK PPQLRSPRLP FRGNSYPGAA EGRAEAPGQP LKPPEGQEGF SQRRGLLSSK
661TYICSHCGES FLDRSVLLQH QLTHGNEKPF LFPDYRIGLG EGAGPSPFLS GKPFKCPECK
721QSFGLSSELL LHQKVHAGGK SSQKSPELGK SSSVLLEHLR SPLGARPYRC SDCRASFLDR
781VALTRHQETH TQEKPPNPED PPPEAVTLST DQEGEGETPT PTESSSHGEG QNPKTLVEEK
841PYLCPECGAG FTEVAALLLH RSCHPGVSL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

869 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.