LSBio
ZNF622 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF622 Protein (Over-Expression Lysate Myc-DDK (Flag))

Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF622 antibodies →
$504.00each
Catalog Number: LS-G102133-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF622 (NM_033414), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 622 | Zinc finger-like protein 9 | ZNF622 | ZPR9
Origin Species
Human
SwissProt
Q969S3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATYTCITCR VAFRDADMQR AHYKTDWHRY NLRRKVASMA PVTAEGFQER VRAQRAVAEE
61ESKGSATYCT VCSKKFASFN AYENHLKSRR HVELEKKAVQ AVNRKVEMMN EKNLEKGLGV
121DSVDKDAMNA AIQQAIKAQP SMSPKKAPPA PAKEARNVVA VGTGGRGTHD RDPSEKPPRL
181QWFEQQAKKL AKQQEEDSEE EEEDLDGDDW EDIDSDEELE CEDTEAMDDV VEQDAEEEEA
241EEGPPLGAIP ITDCLFCSHH SSSLMKNVAH MTKDHSFFIP DIEYLSDIKG LIKYLGEKVG
301VGKICLWCNE KGKSFYSTEA VQAHMNDKSH CKLFTDGDAA LEFADFYDFR SSYPDHKEGE
361DPNKAEELPS EKNLEYDDET MELILPSGAR VGHRSLMRYY KQRFGLSRAV AVAKNRKAVG
421RVLQQYRALG WTGSTGAALM RERDMQYVQR MKSKWMLKTG MKNNATKQMH FRVQVRF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

477 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.