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ZNF621 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF621 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G119205-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF621 (NM_001098414), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 621 | ZNF621
Origin Species
Human
NCBI GenBank
NM_198484
SwissProt
Q6ZSS3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
49 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLQTTWPQES VTFEDVAVYF TQNQWASLDP AQRALYGEVM LENYANVASL VAFPFPKPAL
61ISHLERGEAP WGPDPWDTEI LRGISQGGES WIKNEGLVIK QEASEETELH RMPVGGLLRN
121VSQHFDFKRK ALKQTFNLNP NLILRGGMKF YECKECGKIF RYNSKLIRHQ MSHTGEKPFK
181CKECGKAFKS SYDCIVHEKN HIGEGPYECK ECGKGLSSNT ALTQHQRIHT GEKPYECKEC
241GKAFRRSAAY LQHQRLHTGE KLYKCKECWK AFGCRSLFIV HQRIHTGEKP YQCKECGKAF
301TQKIASIQHQ RVHTGEKPYE CKVCGKAFKW YGSFVQHQKL HPVEKKPVKV LGPSLVSPQC
361SSPAIPPVLL QGSCSASAVA VPSLTFPHAV LIPTSGNFFM LLPTSGIPSS SAQIVRVFQG
421LTPTVKPSPV ILTPSSHSS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

439 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.