LSBio
ZNF618 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF618 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G107544-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF618 (NM_133374), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FP13169 | KIAA1952 | NEDD10 | Zinc finger protein 618 | ZNF618
Origin Species
Human
NCBI GenBank
AA578027
SwissProt
Q5T7W0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
95.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNQPGGAAAP QADGASAAGR KSTASRERLK RSQKSTKVEG PEPVPAEASL SAEQGTMTEV
61KVKTELPDDY IQEVIWQGEA KEEKKAVSKD GTSDVPAEIC VVIGGVRNQQ TLGSYECGIC
121GKKYKYYNCF QTHVRAHRDT EATSGEGASQ SNNFRYTCDI CGKKYKYYSC FQEHRDLHAV
181DVFSVEGAPE NRADPFDQGV VATDEVKEEP PEPFQKIGPM NNITSDIFKK KEVRQCQKRE
241TGNYTCEFCG KQYKYYTPYQ EHVALHAPIK SAFSRRVEGK AQNHFEETNS SSQNSSEPYT
301CGACGIQFQF YNNLLEHMQS HAADNENNIA SNQSRSPPAV VEEKWKPQAQ RNSANNTTTS
361GLTPNSMIPE KERQNIAERL LRVMCADLGA LSVVSGKEFL KLAQTLVDSG ARYGAFSVTE
421ILGNFNTLAL KHLPRMYNQV KVKVTCALGS NACLGIGVTC HSQSVGPDSC YILTAYQAEG
481NHIKSYVLGV KGADIRDSGD LVHHWVQNVL SEFVMSEIRT VYVTDCRVST SAFSKAGMCL
541RCSACALNSV VQSVLSKRTL QARSMHEVIE LLNVCEDLAG STGLAKETFG SLEETSPPPC
601WNSVTDSLLL VHERYEQICE FYSRAKKMNL IQSLNKHLLS NLAAILTPVK QAVIELSNES
661QPTLQLVLPT YVRLEKLFTA KANDAGTVSK LCHLFLEALK ENFKVHPAHK VAMILDPQQK
721LRPVPPYQHE EIIGKVCELI NEVKESWAEE ADFEPAAKKP RSAAVENPAA QEDDRLGKNE
781VYDYLQEPLF QATPDLFQYW SCVTQKHTKL AKLAFWLLAV PAVGARSGCV NMCEQALLIK
841RRRLLSPEDM NKLMFLKSNM L
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

861 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.