LSBio
ZNF615 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF615 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF615 antibodies →
$504.00each
Catalog Number: LS-G103681-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF615 (NM_198480), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001199324

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
83.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMQAQESLTL EDVAVDFTWE EWQFLSPAQK DLYRDVMLEN YSNLVAVGYQ ASKPDALSKL
61ERGEETCTTE DEIYSRICSE IRKIDDPLQH HLQNQSIQKS VKQCHEQNMF GNIVNQNKGH
121FLLKQDCDTF DLHEKPLKSN LSFENQKRSS GLKNSAEFNR DGKSLFHANH KQFYTEMKFP
181AIAKPINKSQ FIKQQRTHNI ENAHVCSECG KAFLKLSQFI DHQRVHTGEK PHVCSMCGKA
241FSRKSRLMDH QRTHTELKHY ECTECDKTFL KKSQLNIHQK THMGGKPYTC SQCGKAFIKK
301CRLIYHQRTH TGEKPHGCSV CGKAFSTKFS LTTHQKTHTG EKPYICSECG KGFIEKRRLT
361AHHRTHTGEK PFICNKCGKG FTLKNSLITH QQTHTGEKLY TCSECGKGFS MKHCLMVHQR
421THTGEKPYKC NECGKGFALK SPLIRHQRTH TGEKPYVCTE CRKGFTMKSD LIVHQRTHTA
481EKPYICNDCG KGFTVKSRLI VHQRTHTGEK PYVCGECGKG FPAKIRLMGH QRTHTGEKPY
541ICNECGKGFT EKSHLNVHRR THTGEKPYVC SECGKGLTGK SMLIAHQRTH TGEKPYICNE
601CGKGFTMKST LSIHQQTHTG EKPYKCNECD KTFRKKTCLI QHQRFHTGKT SFACTECGKF
661SLRKNDLITH QRIHTGEKPY KCSDCGKAFT TKSGLNVHQR KHTGERPYGC SDCGKAFAHL
721SILVKHRRIH R
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

731 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.