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ZNF609 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF609 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF609 antibodies →
$725.00each
Catalog Number: LS-G113512-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF609 (NM_015042), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0295 | Zinc finger protein 609 | ZNF609
Origin Species
Human
NCBI GenBank
NM_015042
SwissProt
O15014

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
151 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSLSSGASGG KGVDANPVET YDSGDEWDIG VGNLIIDLDA DLEKDQQKLE MSGSKEVGIP
61APNAVATLPD NIKFVTPVPG PQGKEGKSKS KRSKSGKDTS KPTPGTSLFT PSEGAASKKE
121VQGRSGDGAN AGGLVAAIAP KGSEKAAKAS RSVAGSKKEK ENSSSKSKKE RSEGVGTCSE
181KDPGVLQPVP LGGRGGQYDG SAGVDTGAVE PLGSIAIEPG AALNPLGTKP EPEEGENECR
241LLKKVKSEKM ESPVSTPAVL PIHLLVPVVN NDISSPCEQI MVRTRSVGVN TCDVALATEP
301ECLGPCEPGT SVNLEGIVWQ ETEDGMLVVN VTWRNKTYVG TLLDCTRHDW APPRFCDSPT
361SDLEMRNGRG RGKRMRPNSN TPVNETATAS DSKGTSNSSK TRAGANSKGR RGSQNSSEHR
421PPASSTSEDV KASPSSANKR KNKPLSDMEL NSSSEDSKGS KRVRTNSMGS ATGPLPGTKV
481EPTVLDRNCP SPVLIDCPHP NCNKKYKHIN GLKYHQAHAH TDDDSKPEAD GDSEYGEEPI
541LHADLGSCNG ASVSQKGSLS PARSATPKVR LVEPHSPSPS SKFSTKGLCK KKLSGEGDTD
601LGALSNDGSD DGPSVMDETS NDAFDSLERK CMEKEKCKKP SSLKPEKIPS KSLKSARPIA
661PAIPPQQIYT FQTATFTAAS PGSSSGLTAT VAQAMPNSPQ LKPIQPKPTV MGEPFTVNPA
721LTPAKDKKKK DKKKKESSKE LESPLTPGKV CRAEEGKSPF RESSGDGMKM EGLLNGSSDP
781HQSRLASIKA EADKIYSFTD NAPSPSIGGS SRLENTTPTQ PLTPLHVVTQ NGAEASSVKT
841NSPAYSDISD AGEDGEGKVD SVKSKDAEQL VKEGAKKTLF PPQPQSKDSP YYQGFESYYS
901PSYAQSSPGA LNPSSQAGVE SQALKTKRDE EPESIEGKVK NDICEEKKPE LSSSSQQPSV
961IQQRPNMYMQ SLYYNQYAYV PPYGYSDQSY HTHLLSTNTA YRQQYEEQQK RQSLEQQQRG
1021VDKKAEMGLK EREAALKEEW KQKPSIPPTL TKAPSLTDLV KSGPGKAKEP GADPAKSVII
1081PKLDDSSKLP GQAPEGLKVK LSDASHLSKE ASEAKTGAEC GRQAEMDPIL WYRQEAEPRM
1141WTYVYPAKYS DIKSEDERWK EERDRKLKEE RSRSKDSVPK EDGKESTSSD CKLPTSEESR
1201LGSKEPRPSV HVPVSSPLTQ HQSYIPYMHG YSYSQSYDPN HPSYRSMPAV MMQNYPGSYL
1261PSSYSFSPYG SKVSGGEDAD KARASPSVTC KSSSESKALD ILQQHASHYK SKSPTISDKT
1321SQERDRGGCG VVGGGGSCSS VGGASGGERS VDRPRTSPSQ RLMSTHHHHH HLGYSLLPAQ
1381YNLPYAAGLS STAIVASQQG STPSLYPPPR R
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1411 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.