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ZNF596 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF596 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G119499-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF596 (NM_001042416), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZNF596 | Zinc finger protein 596
Origin Species
Human
NCBI GenBank
NM_173539
SwissProt
Q8TC21

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPSPDSMTFE DIIVDFTQEE WALLDTSQRK LFQDVMLENI SHLVSIGKQL CKSVVLSQLE
61QVEKLSTQRI SLLQGREVGI KHQEIPFIHH IYQKGTSTIS TMRSHTQEDP FLCNDLGEDF
121TQHIALTQNV ITYMRTKHFV SKKFGKIFSD WLSFNQHKEI HTKCKSYGSH LFDYAFIQNS
181ALRPHSVTHT REITLECRVC GKTFSKNSNL RRHEMIHTGE KPHGCHLCGK AFTHCSDLRK
241HERTHTGEKP YGCHLCGKAF SKSSNLRRHE MIHTREKAQI CHLCGKAFTH CSDLRKHERT
301HLGDKPYGCL LCGKAFSKCS YLRQHERTHN GEKPYECHLC GKAFSHCSHL RQHERSHNGE
361KPHGCHLCGK AFTESSVLKR HERIHTGEKP YECHVCGKAF TESSDLRRHE RTHTGEKPYE
421CHLCGKAFNH SSVLRRHERT HTGEKPYECN ICGKAFNRSY NFRLHRRVHT GEKPYVCPLC
481GKAFSKFFNL RQHERTHTKK AMNM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

504 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.