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ZNF585B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF585B Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF585B antibodies →
$504.00each
Catalog Number: LS-G106421-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF585B (NM_152279), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SZFP41 | ZNF585B | Zinc finger protein 585B
Origin Species
Human
SwissProt
Q52M93

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
87.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPASWTSPQK SSALAPEDHG SSYEGSVSFR DVAIDFSREE WRHLDLSQRN LYRDVMLETY
61SHLLSVGYQV PKPEVVMLEQ GKEPWALQGE RPRHSCPGEK LWDHNQHRKI IGYKPASSQD
121QKIYSGEKSY ECAEFGKSFT WKSQFKVHLK VPTGEKLYVC IECGRAFVQK PEFITHQKTH
181MREKPYKCNE CGKSFFQVSS LFRHHRIHTG EKLYECSECG KGFPYNSDLS IHEKIHTGER
241HHECTDCGKA FTQKSTLKIH QKIHTGERSY ICIECGQAFI QKTQLIAHRR IHSGEKPYEC
301NNCGKSFISK SQLQVHQRVH TRVKPYICTE YGKVFSNNSN LITHEKIQSR EKSSICTECG
361KAFTYRSELI IHQRIHTGEK PYECSDCGRA FTQKSALTVH QRIHTGEKSY ICMKCGLAFI
421RKAHLITHQI IHTGEKPYKC GHCGKLFTSK SQLHVHKRIH TGEKPYVCNK CGKAFTNRSN
481LITHQKTHTG EKSYICSKCG KAFTQRSDLI THQRIHTGEK PYECNTCGKA FTQKSNLNIH
541QKIHTGERQY ECHECGKAFN QKSILIVHQK IHTGEKPYVC TECGRAFIRK SNFITHQRIH
601TGEKPYECSD CGKSFTSKSQ LLVHQPVHTG EKPYVCAECG KAFSGRSNLS KHQKTHTGEK
661PYICSECGKT FRQKSELITH HRIHTGEKPY ECSDCGKSFT KKSQLQVHQR IHTGEKPYVC
721AECGKAFSNR SNLNKHQTTH TGDKPYKCGI CGKGFVQKSV FSVHQSSHA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

769 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.