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ZNF583 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF583 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G106256-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF583 (NM_152478), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 583 | Zinc finger protein L3-5 | ZNF583
Origin Species
Human
SwissProt
Q96ND8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSKDLVTFGD VAVNFSQEEW EWLNPAQRNL YRKVMLENYR SLVSLGVSVS KPDVISLLEQ
61GKEPWMVKKE GTRGPCPDWE YVFKNSEFSS KQETYEESSK VVTVGARHLS YSLDYPSLRE
121DCQSEDWYKN QLGSQEVHLS QLIITHKEIL PEVQNKEYNK SWQTFHQDTI FDIQQSFPTK
181EKAHKHEPQK KSYRKKSVEM KHRKVYVEKK LLKCNDCEKV FNQSSSLTLH QRIHTGEKPY
241ACVECGKTFS QSANLAQHKR IHTGEKPYEC KECRKAFSQN AHLAQHQRVH TGEKPYQCKE
301CKKAFSQIAH LTQHQRVHTG ERPFECIECG KAFSNGSFLA QHQRIHTGEK PYVCNVCGKA
361FSHRGYLIVH QRIHTGERPY ECKECRKAFS QYAHLAQHQR VHTGEKPYEC KVCRKAFSQI
421AYLDQHQRVH TGEKPYECIE CGKAFSNSSS LAQHQRSHTG EKPYMCKECR KTFSQNAGLA
481QHQRIHTGEK PYECNVCGKA FSYSGSLTLH QRIHTGERPY ECKDCRKSFR QRAHLAHHER
541IHTMESFLTL SSPSPSTSNQ LPRPVGFIS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

569 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.