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ZNF582 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF582 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G106897-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF582 (NM_144690), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 582 | ZNF582
Origin Species
Human
SwissProt
Q96NG8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MSLGSELFRD VAIVFSQEEW QWLAPAQRDL YRDVMLETYS NLVSLGLAVS KPDVISFLEQ
61GKEPWMVERV VSGGLCPVLE SRYDTKELFP KQHVYEVESP QWEIMESLTS YGLECSSFQD
121DWECRNQFDR QQGNPDRHFH QMIIRHEEMP TFDQHASLTF YQKIHTREKP FGYNKCRKDF
181WQKELLINHQ GIYTNEKPYK CKECGKAFKY GSRLIQHENI HSGKKPYECK ECGKAFNSGS
241NFIQHQRVHT GEKPYECKDC EKAFSRSSQL IEHQRTHTGE KPYQCKECGK AFNRISHLKV
301HYRIHTGEKP YACKECGKTF SHRSQLIQHQ TVHTGRKLYE CKECGKAFNQ GSTLIRHQRI
361HTGEKPYECK VCGKAFRVSS QLKQHQRIHT GEKPYQCKVC GRAFKRVSHL TVHYRIHTGE
421KPYECKECGK AFSHCSQLIH HQVIHTEKKP YEYKECEKTL SHDSTTVQPQ RMHNRETHVN
481IINVEKPSIS SYPLLIIREF MLASNHMNGS NGESPLA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

517 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.