LSBio
ZNF579 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF579 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF579 antibodies →
$725.00each
Catalog Number: LS-G106132-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF579 (NM_152600), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 579 | ZNF579
Origin Species
Human
SwissProt
Q8NAF0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MDPQPPPPAQ GSPPHRGRGR GRGRGRGRGR GRGRGGAGAP RAPLPCPTCG RLFRFPYYLS
61RHRLSHSGLR PHACPLCPKA FRRPAHLSRH LRGHGPQPPL RCAACPRTFP EPAQLRRHLA
121QEHAGGEVEL AIERVAKETA EPSWGPQDEG SEPPTTAAAG ATEEEAVAAW PETWPAGEPS
181TLAAPTSAAE PRESESEEAE AGAAELRAEL ALAAGRQEEK QVLLQADRTL LCLRCREAFA
241TKGELKAHPC LRPEGEQEGE GGPPPRPKRH QCSICLKAFA RPWSLSRHRL VHSTDRPFVC
301PDCGLAFRLA SYLRQHRRVH GPLSLLAPLP AAGKKDDKAS GARNSAKGPE GGEGAECGGA
361SEGGEGQNGG DAAPARPPAG EPRFWCPECG KGFRRRAHLR QHGVTHSGAR PFQCVRCQRE
421FKRLADLARH AQVHAGGPAP HPCPRCPRRF SRAYSLLRHQ RCHRAELERA AALQALQAQA
481PTSPPPPPPP LKAEQEEEGL PLPLANIKEE PPSPGTPPQS PPAPPVFLSA SCFDSQDHSA
541FEMEEEEVDS KAHLRGLGGL AS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

562 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.