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ZNF574 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF574 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G110276-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF574 (NM_022752), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FP972 | Zinc finger protein 574 | ZNF574
Origin Species
Human
NCBI GenBank
NM_022752
SwissProt
Q6ZN55

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
98.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTEESEETVL YIEHRYVCSE CNQLYGSLEE VLMHQNSHVP QQHFELVGVA DPGVTVATDT
61ASGTGLYQTL VQESQYQCLE CGQLLMSPSQ LLEHQELHLK MMAPQEAVPA EPSPKAPPLS
121SSTIHYECVD CKALFASQEL WLNHRQTHLR ATPTKAPAPV VLGSPVVLGP PVGQARVAVE
181HSYRKAEEGG EGATVPSAAA TTTEVVTEVE LLLYKCSECS QLFQLPADFL EHQATHFPAP
241VPESQEPALQ QEVQASSPAE VPVSQPDPLP ASDHSYELRN GEAIGRDRRG RRARRNNSGE
301AGGAATQELF CSACDQLFLS PHQLQQHLRS HREGVFKCPL CSRVFPSPSS LDQHLGDHSS
361ESHFLCVDCG LAFGTEALLL AHRRAHTPNP LHSCPCGKTF VNLTKFLYHR RTHGVGGVPL
421PTTPVPPEEP VIGFPEPAPA ETGEPEAPEP PVSEETSAGP AAPGTYRCLL CSREFGKALQ
481LTRHQRFVHR LERRHKCSIC GKMFKKKSHV RNHLRTHTGE RPFPCPDCSK PFNSPANLAR
541HRLTHTGERP YRCGDCGKAF TQSSTLRQHR LVHAQHFPYR CQECGVRFHR PYRLLMHRYH
601HTGEYPYKCR ECPRSFLLRR LLEVHQLVVH AGRQPHRCPS CGAAFPSSLR LREHRCAAAA
661AQAPRRFECG TCGKKVGSAA RLQAHEAAHA AAGPGEVLAK EPPAPRAPRA TRAPVASPAA
721LGSTATASPA APARRRGLEC SECKKLFSTE TSLQVHRRIH TGERPYPCPD CGKAFRQSTH
781LKDHRRLHTG ERPFACEVCG KAFAISMRLA EHRRIHTGER PYSCPDCGKS YRSFSNLWKH
841RKTHQQQHQA AVRQQLAEAE AAVGLAVMET AVEALPLVEA IEIYPLAEAE GVQISG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

896 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.