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ZNF571 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF571 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G112594-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF571 (NM_016536), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 571 | HSPC059 | ZNF571
Origin Species
Human
NCBI GenBank
NM_016536
SwissProt
Q7Z3V5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPHLLVTFRD VAIDFSQEEW ECLDPAQRDL YRDVMLENYS NLISLDLESS CVTKKLSPEK
61EIYEMESLQW ENMGKRINHH LQYNGLGDNM ECKGNLEGQE ASQEGLYMCV KITCEEKATE
121SHSTSSTFHR IIPTKEKLYK CKECRQGFSY LSCLIQHEEN HNIEKCSEVK KHRNTFSKKP
181SYIQHQRIQT GEKPYECMEC GKAFGRTSDL IQHQKIHTNE KPYQCNACGK AFIRGSQLTE
241HQRVHTGEKP YDCKKCGKAF SYCSQYTLHQ RIHSGEKPYE CKDCGKAFIL GSQLTYHQRI
301HSGEKPYECK ECGKAFILGS HLTYHQRVHT GEKPYICKEC GKAFLCASQL NEHQRIHTGE
361KPYECKECGK TFFRGSQLTY HLRVHSGERP YKCKECGKAF ISNSNLIQHQ RIHTGEKPYK
421CKECGKAFIC GKQLSEHQRI HTGEKPFECK ECGKAFIRVA YLTQHEKIHG EKHYECKECG
481KTFVRATQLT YHQRIHTGEK PYKCKECDKA FIYGSQLSEH QRIHRGEKPY ECKQCGKAFI
541RGSHLTEHLR THTGEKPYEC KECGRAFSRG SEHTLHQRIH TGEKPYTCVQ CGKDFRCPSQ
601LTQHTRLHN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

609 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.