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ZNF57 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF57 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G105328-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF57 (NM_173480), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 57 | ZNF57 | Zinc finger protein 424 | ZNF424
Origin Species
Human
SwissProt
Q68EA5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDSVVFEDVA VDFTLEEWAL LDSAQRDLYR DVMLETFRNL ASVDDGTQFK ANGSVSLQDM
61YGQEKSKEQT IPNFTGNNSC AYTLEKNCEG YGTEDHHKNL RNHMVDRFCT HNEGNQYGEA
121IHQMPDLTLH KKVSAGEKPY ECTKCRTVFT HLSSLKRHVK SHCGRKAPPG EECKQACICP
181SHLHSHGRTD TEEKPYKCQA CGQTFQHPRY LSHHVKTHTA EKTYKCEQCR MAFNGFASFT
241RHVRTHTKDR PYKCQECGRA FIYPSTFQRH MTTHTGEKPY KCQHCGKAFT YPQAFQRHEK
301THTGEKPYEC KQCGKTFSWS ETLRVHMRIH TGDKLYKCEH CGKAFTSSRS FQGHLRTHTG
361EKPYECKQCG KAFTWSSTFR EHVRIHTQEQ LYKCEQCGKA FTSSRSFRGH LRTHTGEKPY
421ECKQCGKTFT WSSTFREHVR IHTQEQLHKC EHCGKAFTSS RAFQGHLRMH TGEKPYECKQ
481CGKTFTWSST LHNHVRMHTG EKPHKCKQCG MSFKWHSSFR NHLRMHTGQK SHECQSYSKA
541FSCQVILSKT SESTH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

555 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.