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ZNF565 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF565 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G119537-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF565 (NM_001042474), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 565 | ZNF565
Origin Species
Human
NCBI GenBank
AA448909
SwissProt
Q8N9K5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAQGLVTFRD VAIEFSLEEW KCLEPAQRDL YREVTLENFG HLASLGLSIS KPDVVSLLEQ
61GKEPWMIAND VTGPWCPDLE SRCEKFLQKD IFEIGAFNWE IMESLKCSDL EGSDFRADWE
121CEGQFERQVN EECYFKQVNV TYGHMPVFQH HTSHTVRQSR ETGEKLMECH ECGKAFSRGS
181HLIQHQKTHT GEKPFGCKEC GKAFSRASHL VQHQRIHTGE KPYDCKDCGK AFGRTSELIL
241HQRLHTGVKP YECKECGKTF RQHSQLILHQ RTHTGEKPYV CKDCGKAFIR GSQLTVHRRI
301HTGARPYECK ECGKAFRQHS QLTVHQRIHT GEKPYECKEC GKGFIHSSEV TRHQRIHSGE
361KPYECKECGK AFRQHAQLTR HQRVHTGDRP YECKDCGKAF SRSSYLIQHQ RIHTGDKPYE
421CKECGKAFIR VSQLTHHQRI HTCEKPYECR ECGMAFIRSS QLTEHQRIHP GIKPYECREC
481GQAFILGSQL IEHYRIHTG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

499 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.