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ZNF555 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF555 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G106009-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF555 (NM_152791), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 555 | ZNF555
Origin Species
Human
NCBI GenBank
NM_152791

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDSVVFEDVA VDFTLEEWAL LDSAQRDLYR DVMLETFQNL ASVDDETQFK ASGSVSQQDI
61YGEKIPKESK IATFTRNVSW ASVLGKIWDS LSIEDQTTNQ GRNLSRDHGL ERLCESNDQC
121GEALSQIPHL NLYKKIPPGV KQYEYNTYGK VFMHRRTSLK SPITVHTGHK PYQCQECGQA
181YSCRSHLRMH VRTNNGERPY VCKLCGKTFP RTSSLNRHVR IHTAEKTYEC KQCGKAFIDF
241SSLTSHLRSH TGEKPYKCKE CGKAFSYSST FRRHTITHTG EKPYKCKECA EAFSYSSTFR
301RHMISHTGEK PHKCKECGEA FSYSSAFRRH MITHTGEKPY ECKQCGKTFI YLQSFRRHER
361IHTGEKPYEC KQCGKTFIYP QSFRRHERTH GGEKPYECNQ CGKAFSHPSS FRGHMRVHTG
421EKPYECKQCG KTFNWPISLR KHMRTHTREK PYECKQCGKA FSLSACFREH VRMHPEDKSY
481ECKLCGKAFY CHISLQKHMR RHTAEKLYKC KQCGTAFSWP ELLQQHVRTH TVEKPYECKE
541CGKVFKWPSS LPIHMRLHTG EKPYQCKHCG KAFNCSSSLR RHVRIHTTEK QYKCNVGHPP
601ANEFMCSASE KSHQERDLIK VVNMVLPL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

628 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.