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ZNF549 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF549 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G105843-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF549 (NM_153263), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZNF549 | Zinc finger protein 549
Origin Species
Human
SwissProt
Q6P9A3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEAALVNTP QGHVTFEDIA VYFSQEEWGL LDEAQRCLYH DVMLENFSLM ASVGCLHGIE
61AEEAPSEQTL SAQGVSQART PKLGPSIPNA HSCEMCILVM KDILYLSEHQ GTLPWQKPYT
121SVASGKWFSF GSNLQQHQNQ DSGEKHIRKE ESSALLLNSC KIPLSDNLFP CKDVEKDFPT
181ILGLLQHQTT HSRQEYAHRS RETFQQRRYK CEQVFNEKVH VTEHQRVHTG EKAYKRREYG
241KSLNSKYLFV EHQRTHNAEK PYVCNICGKS FLHKQTLVGH QQRIHTRERS YVCIECGKSL
301SSKYSLVEHQ RTHNGEKPYV CNVCGKSFRH KQTFVGHQQR IHTGERPYVC MECGKSFIHS
361YDRIRHQRVH TGEGAYQCSE CGKSFIYKQS LLDHHRIHTG ERPYECKECG KAFIHKKRLL
421EHQRIHTGEK PYVCIICGKS FIRSSDYMRH QRIHTGERAY ECSDCGKAFI SKQTLLKHHK
481IHTRERPYEC SECGKGFYLE VKLLQHQRIH TREQLCECNE CGKVFSHQKR LLEHQKVHTG
541EKPCECSECG KCFRHRTSLI QHQKVHSGER PYNCTACEKA FIYKNKLVEH QRIHTGEKPY
601ECGKCGKAFN KRYSLVRHQK VHITEEP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

627 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.