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ZNF543 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF543 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G102705-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF543 (NM_213598), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 543 | ZNF543
Origin Species
Human
NCBI GenBank
NM_213598
SwissProt
Q08ER8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAASAQVSVT FEDVAVTFTQ EEWGQLDAAQ RTLYQEVMLE TCGLLMSLGC PLFKPELIYQ
61LDHRQELWMA TKDLSQSSYP GDNTKPKTTE PTFSHLALPE EVLLQEQLTQ GASKNSQLGQ
121SKDQDGPSEM QEVHLKIGIG PQRGKLLEKM SSERDGLGSD DGVCTKITQK QVSTEGDLYE
181CDSHGPVTDA LIREEKNSYK CEECGKVFKK NALLVQHERI HTQVKPYECT ECGKTFSKST
241HLLQHLIIHT GEKPYKCMEC GKAFNRRSHL TRHQRIHSGE KPYKCSECGK AFTHRSTFVL
301HHRSHTGEKP FVCKECGKAF RDRPGFIRHY IIHTGEKPYE CIECGKAFNR RSYLTWHQQI
361HTGVKPFECN ECGKAFCESA DLIQHYIIHT GEKPYKCMEC GKAFNRRSHL KQHQRIHTGE
421KPYECSECGK AFTHCSTFVL HKRTHTGEKP YECKECGKAF SDRADLIRHF SIHTGEKPYE
481CVECGKAFNR SSHLTRHQQI HTGEKPYECI QCGKAFCRSA NLIRHSIIHT GEKPYECSEC
541GKAFNRGSSL THHQRIHTGR NPTIVTDVGR PFMTAQTSVN IQELLLGKEF LNITTEENLW
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

600 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.