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ZNF541 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF541 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF541 antibodies →
$725.00each
Catalog Number: LS-G124056-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF541 (NM_001101419), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 541 | ZNF541
Origin Species
Human
NCBI GenBank
NM_032255
SwissProt
Q9H0D2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
147.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDQYSLGDEG ALPSEMHLPS FSESQGLNCS DTLNRDLGPN TRGFLYAGLS GLDPDPSLPT
61PDMSSEVLED NLDTLSLYSG KDSDSVKLLE EYADSESQAS LQDLGLGVLK AKEADEGGRA
121TSGSARKGKR QHSSPQNPLL DCSLCGKVFS SASSLSKHYL THSQERKHVC KICSKAFKRQ
181DHLTGHMLTH QKTKPFVCIE QGCSKSYCDY RSLRRHYEVH HGLCILKEAP PEEEACGDSP
241HAHESAGQPP PSSLRSLVPP EARSPGSLLP HRDLLRRIVS SIVHQKTPSP GPAPAGASDS
301EGRNTACPCP ASSGSSSCTP AGPHAAPAAL DTELPEEPCL PQKEPATDVF TAPNSRAAEN
361GAPDPPEPEP DTALLQARST AECWPEGGSV PACLPLFRGQ TVPASSQPSS HSFQWLRNLP
421GCPKSKGNNV FVVHKPSAVP SREGSESGPG PSSGSPSEES PPGPGGGLED ALPFPAALLR
481VPAEAPSDPR SASGEDDPCA PKKVKVDCDS FLCQNPGEPG LQEAQKAGGL PADASPLFRQ
541LFLKSQEPLV SHEQMQVFQM ITKSQRIFSH AQVAAVSSQL PAPEGKPAAL RPLQGPWPQQ
601PPPLAPAVDS LHAGPGNPEA EGSPARRRKT TPGVPREASP GSTRRDAKGG LKVAAVPTPL
661AAPSLDPSRN PDISSLAKQL RSSKGTLDLE DIFPSTGQRQ TQLGGEEPPG ASLPGKQAPA
721ENGAASRITK GEKGPACSRG GGYRLLGNPR APRFSGFRKE KAKMDMCCAA SPSQVAMASF
781SSAGPPADPS KSKLTIFSRI QGGNIYRLPH PVKEENVAGR GNQQNGSPTD WTKPRSTFVC
841KNCSQMFYTE KGLSSHMCFH SDQWPSPRGK QEPQVFGTEF CKPLRQVLRP EGDRHSPPGT
901KKPLDPTAAA PLVVPQSIPV VPVTRHIGSM AMMEWCSRWL QATTCGSKLL QGQEKDGEER
961DSKESSQQRK RKKRPPPSTA GEPGPAGCHQ SRLRSPMFLV DCLLKGLFQC SPYTPPPMLS
1021PIREGSGVYF NTLCSTSTQA SPDQLISSML DQVDGSFGIC VVKDDTKISI EPHINIGSRF
1081QAEIPELQER SLAGTDEHVA SLVWKPWGDM MISSETQDRV TELCNVACSS VMPGGGTNLE
1141LALHCLHEAQ GNVQVALETL LLRGPHKPRT HLLADYRYTG SDVWTPIEKR LFKKAFYAHK
1201KDFYLIHKMI QTKTVAQCVE YYYIWKKMIK FDCGRAPGLE KRVKREPEEV ERTEEKVPCS
1261PRERPSHHPT PKLKTKSYRR ESILSSSPNA GSKRTPELLG SAESQGIFPC RECERVFDKI
1321KSRNAHMKRH RLQDHVEPII RVKWPVKPFQ LKEEELGADI GPLQW
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1365 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.