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ZNF536 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF536 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF536 antibodies →
$725.00each
Catalog Number: LS-G113757-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF536 (NM_014717), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0390 | Zinc finger protein 536 | ZNF536
Origin Species
Human
NCBI GenBank
AB002388
SwissProt
O15090

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
141.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEEASLCLGV SSAEPEAEPH LSGPVLNGQY AMSQKLHQIT SQLSHAFPEL HPRPNPEEKP
61PASLEEKAHV PMSGQPMGSQ MALLANQLGR EVDTSLNGRV DLQQFLNGQN LGIMSQMSDI
121EDDARKNRKY PCPLCGKRFR FNSILSLHMR THTGEKPFKC PYCDHRAAQK GNLKIHLRTH
181KLGNLGKGRG RVREENRLLH ELEERAILRD KQLKGSLLQP RPDLKPPPHA QQAPLAACTL
241ALQANHSVPD VAHPVPSPKP ASVQEDAVAP AAGFRCTFCK GKFKKREELD RHIRILHKPY
301KCTLCDFAAS QEEELISHVE KAHITAESAQ GQGPNGGGEQ SANEFRCEVC GQVFSQAWFL
361KGHMRKHKDS FEHCCQICGR RFKEPWFLKN HMKVHLNKLS VKNKSPSDPE VPVPMGGMSQ
421EAHANLYSRY LSCLQSGFMT PDKAGLSEPS QLYGKGELPM KEKEALGKLL SPISSMAHGV
481PEGDKHSLLG CLNLVPPLKS SCIERLQAAA KAAEMDPVNS YQAWQLMARG MAMEHGFLSK
541EHPLQRNHED TLANAGVLFD KEKREYVLVG ADGSKQKMPA DLVHSTKVGS QRDLPSKLDP
601LESSRDFLSH GLNQTLEYNL QGPGNMKEKP TECPDCGRVF RTYHQVVVHS RVHKRDRKGE
661EDGLHVGLDE RRGSGSDQES QSVSRSTTPG SSNVTEESGV GGGLSQTGSA QEDSPHPSSP
721SSSDIGEEAG RSAGVQQPAL LRDRSLGSAM KDCPYCGKTF RTSHHLKVHL RIHTGEKPYK
781CPHCDYAGTQ SASLKYHLER HHRERQNGAG PLSGQPPNQD HKDEMSSKAS LFIRPDILRG
841AFKGLPGIDF RGGPASQQWT SGVLSSGDHS GQATGMSSEV PSDALKGTDL PSKSTHFSEI
901GRAYQSIVSN GVNFQGSLQA FMDSFVLSSL KKEKDMKDKA LADPPSMKVH GVDGGEEKPS
961GKSSQRKSEK SQYEPLDLSV RPDAASLPGS SVTVQDSIAW HGCLFCAFTT SSMELMALHL
1021QANHLGKAKR KDNTIGVTVN CKDQAREASK MALLPSLQSN KDLGLSNMIS SLDSASEKMA
1081QGQLKETLGE QKSGAWTGHV DPAFCNFPSD FYKQFGVYPG MVGSGASSSC PNKEPDGKAH
1141SEEDVPILIP ETTSKNTTDD LSDIASSEDM DSSKGENNDE EDVETEPEMM TKPLSALSKD
1201SSSDGGDSLQ PTGTSQPVQG LVSPLSQAPE KQWHSQGLLQ AQDPLAGLPK PERGPQSLDK
1261PMNMLSVLRA YSSDGLAAFN GLASSTANSG CIKRPDLCGK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1300 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.