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ZNF529 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF529 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G110862-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF529 (NM_020951), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1615 | ZNF529 | Zinc finger protein 529
Origin Species
Human
NCBI GenBank
NM_020951
SwissProt
Q6P280

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.5 kda
Protein Type
Lysate
Amino Acid Sequence
1MDHELVTLRD VVINFSQEEW EYLDSAQRNL YWDVMMENYS NLLSLDLESR NETKHLSVGK
61DIIQNTGPQW EVMESSKLCG LEGSIFRNDW QSKSKIDVQG PEVGYFSQMK IISENVPSYK
121THESLTLPRR THDSEKPYEY KEYEKVFSCD LEFDEYQKIH TGGKNYECNQ CWKTFGIDNS
181SMLQLNIHTG VKPCKYMEYG NTCSFYKDFN VYQKIHNEKF YKCKEYRRTF ERVGKVTPLQ
241RVHDGEKHFE CSFCGKSFRV HAQLTRHQKI HTDEKTYKCM ECGKDFRFHS QLTEHQRIHT
301GEKPYKCMHC EKVFRISSQL IEHQRIHTGE KPYACKECGK AFGVCRELAR HQRIHTGKKP
361YECKACGKVF RNSSSLTRHQ RIHTGEKPYK CKECEKAFGV GSELTRHERI HSGQKPYECK
421ECGKFFRLTS ALIQHQRIHS GEKPYECKVC GKAFRHSSAL TEHQRIHTGE KPYECKACGK
481AFRHSSSFTK HQRIHTDDKP YECKECGNSF SVVGHLTCQP KIYTGEKSFD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

530 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.