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ZNF526 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF526 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G107492-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF526 (NM_133444), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZNF526 | KIAA1951 | Zinc finger protein 526
Origin Species
Human
SwissProt
Q8TF50

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
73.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEVVAEVAE MPTQMSPGAV EMSTPMSAEM MEMSTEVTEM TPGEALASSL FFQHHQFMCS
61ECGSLYNTLE EVLSHQEQHM LAVSEEEALT TQNVGLEPEL VPGAEGPFQC GECSQLILSP
121GELLAHQDAH LRESANQIQY QCWDCQELFP SPELWVAHRK AQHLSATVAE PPVPPPLPPP
181TPLPPPSPPS EVKMEPYECP ECSTLCATPE EFLEHQGTHF DSLEKEERNG LEEEEEDDEE
241DEEDDEEMED EEAMAEVGDD AVGGDESTAG WAQGCGDCPQ HQPSAGARRQ HRRTAHSPAS
301ATHPFHCSQC QRSFSSANRL QAHGRAHVGG THECTTCSKV FKKAASLEQH LRLHRGEARY
361LCVDCGRGFG TELTLVAHRR AHTANPLHRC RCGKTFSNMT KFLYHRRTHA GKSGAPPTGA
421TAPPAPAEPT PPPPPPAPPA QLPCPQCSKS FASASRLSRH RRAVHGPPER RHRCGVCGKG
481FKKLIHVRNH LRTHTGERPF QCHSCGKTFA SLANLSRHQL THTGARPYQC LDCGKRFTQS
541SNLQQHRRLH LRPVAFARAP RLPITGLYNK SPYYCGTCGR WFRAMAGLRL HQRVHARART
601LTLQPPRSPS PAPPPPPEPQ QTIMCTELGE TIAIIETSQP LALEDTLQLC QAALGASEAG
661GLLQLDTAFV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

670 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.