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ZNF521 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF521 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF521 antibodies →
$725.00each
Catalog Number: LS-G101335-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF521 (NM_015461), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Evi3 | EHZF | Zinc finger protein 521 | ZNF521 | LIP3 | LYST-interacting protein 3
Origin Species
Human
NCBI GenBank
AA461407
SwissProt
Q96K83

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
147.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSRRKQAKPR SLKDPNCKLE DKTEDGEALD CKKRPEDGEE LEDEAVHSCD SCLQVFESLS
61DITEHKINQC QLTDGVDVED DPTCSWPASS PSSKDQTSPS HGEGCDFGEE EGGPGLPYPC
121QFCDKSFSRL SYLKHHEQSH SDKLPFKCTY CSRLFKHKRS RDRHIKLHTG DKKYHCSECD
181AAFSRSDHLK IHLKTHTSNK PYKCAICRRG FLSSSSLHGH MQVHERNKDG SQSGSRMEDW
241KMKDTQKCSQ CEEGFDFPED LQKHIAECHP ECSPNEDRAA LQCVYCHELF VEETSLMNHM
301EQVHSGEKKN SCSICSESFH TVEELYSHMD SHQQPESCNH SNSPSLVTVG YTSVSSTTPD
361SNLSVDSSTM VEAAPPIPKS RGRKRAAQQT PDMTGPSSKQ AKVTYSCIYC NKQLFSSLAV
421LQIHLKTMHL DKPEQAHICQ YCLEVLPSLY NLNEHLKQVH EAQDPGLIVS AMPAIVYQCN
481FCSEVVNDLN TLQEHIRCSH GFANPAAKDS NAFFCPHCYM GFLTDSSLEE HIRQVHCDLS
541GSRFGSPVLG TPKEPVVEVY SCSYCTNSPI FNSVLKLNKH IKENHKNIPL ALNYIHNGKK
601SRALSPLSPV AIEQTSLKMM QAVGGAPARP TGEYICNQCG AKYTSLDSFQ THLKTHLDTV
661LPKLTCPQCN KEFPNQESLL KHVTIHFMIT STYYICESCD KQFTSVDDLQ KHLLDMHTFV
721FFRCTLCQEV FDSKVSIQLH LAVKHSNEKK VYRCTSCNWD FRNETDLQLH VKHNHLENQG
781KVHKCIFCGE SFGTEVELQC HITTHSKKYN CKFCSKAFHA IILLEKHLRE KHCVFETKTP
841NCGTNGASEQ VQKEEVELQT LLTNSQESHN SHDGSEEDVD TSEPMYGCDI CGAAYTMETL
901LQNHQLRDHN IRPGESAIVK KKAELIKGNY KCNVCSRTFF SENGLREHMQ THLGPVKHYM
961CPICGERFPS LLTLTEHKVT HSKSLDTGNC RICKMPLQSE EEFLEHCQMH PDLRNSLTGF
1021RCVVCMQTVT STLELKIHGT FHMQKTGNGS AVQTTGRGQH VQKLYKCASC LKEFRSKQDL
1081VKLDINGLPY GLCAGCVNLS KSASPGINVP PGTNRPGLGQ NENLSAIEGK GKVGGLKTRC
1141SSCNVKFESE SELQNHIQTI HRELVPDSNS TQLKTPQVSP MPRISPSQSD EKKTYQCIKC
1201QMVFYNEWDI QVHVANHMID EGLNHECKLC SQTFDSPAKL QCHLIEHSFE GMGGTFKCPV
1261CFTVFVQANK LQQHIFSAHG QEDKIYDCTQ CPQKFFFQTE LQNHTMTQHS S
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1311 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.