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ZNF518B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF518B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF518B antibodies →
$725.00each
Catalog Number: LS-G108040-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF518B (NM_053042), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZNF518B | Zinc finger protein 518B | KIAA1729
Origin Species
Human
NCBI GenBank
NM_053042
SwissProt
Q9C0D4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
119.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKDIGQQLYT THLNGGHNSL TMSPKQPDAN GAPRPNRQEA QTLLYQGSEA EAAMMTIATC
61AKCKSVHKIS LQDLQKGTGK DGMYVCFQCS LGAAPPNFHF VSNNSSATHV GNKTENFSSS
121VNSKFKVRNF KPGKYYCDKC RFSTKDPLQY KKHTLQHEEI KFICSHCSYI SYTKGEFQRH
181LVKHTGIFPY QCEYCDYGAI RNDYIVKHTK RVHERAGAKR PVKAVAKLEP KRTGTSKQNP
241ELLKASNPRT TFQNKWSDQL SGFSLHANKD KMHNIMLLPE PKEYQKDVVC IPNKMTLSEP
301NEVNLFENKN VEVEVLSPAK EPVQPGMPLT VVAPAELVVP ANCLAQLIDV KVVNGTQQLV
361LKLFPLEENN CLEAGRDNGG NSERMVKEKG SNEQEKVLSA EKTKSLTVDG NVGKLVGIDS
421FQPSVQKQLK NVKWVRSYDF IMPNSSVHNN GKSFINSETI EDFQKKNNLY PHRTAFPSVA
481LKGHSLASVF KNSVLRSLGA ASNPFPYKAA VCFAESGRNL HSSSQQLLPF AASPATCSFS
541GEKGLLPVSE NDLESTSKVN IPVKVVSSNR KQEDNQTEEH KAVSTVGQIS SQHKSEYLHI
601NITGEDRSQQ PGDKPLELKN SERTNNTNDG PVISSVFSLS SGSENVPEGI KWNSSTSKIK
661SIELLRRKIA QLIESCGKPS SLASNSAHRR SVGQASKGTS KATSEGIQEI NVSLTGLGHS
721TGTLQKPPND GGITGNRQLT HQQIYPHFAD GSNRKTKSRV ARKAHVATPV LIPKGAVLRV
781LNSSENAHII EATCEAPVSI PCSERQLIKP VPFCPVRQAD SDLQPLRSER GPIDMSPNIE
841TPLRPKLRKE SAVCSTIHRK TGLLYGQQGS SELNKQGRLL SRSLSISRNK TKQVHLSRKK
901NKIQAEPSRC LKDPSIFQVA RQLRLIAAKP DQLIKCPRRN QPVIVLNHPD VDSPEVTNVM
961KVINKYKGNV LKVVLSERTR CQLGIRRHHV RLTYQNAEEA SQIKRQMMLK MKLKKVHKNN
1021YQVVDSLPDD SSQCVFKCWF CGRLYEDQEE WMSHGQRHLI EATRDWDVLS SKGK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1074 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.