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ZNF516 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF516 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZNF516 antibodies →
$725.00each
Catalog Number: LS-G113813-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF516 (NM_014643), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZNF516 | Zinc finger protein 516 | HsT287 | KIAA0222
Origin Species
Human
NCBI GenBank
D86975
SwissProt
Q92618

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
124.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDRNREAEME LRRGPSPTRA GRGHEVDGDK ATCHTCCICG KSFPFQSSLS QHMRKHTGEK
61PYKCPYCDHR ASQKGNLKIH IRSHRTGTLI QGHEPEAGEA PLGEMRASEG LDACASPTKS
121ASACNRLLNG ASQADGARVL NGASQADSGR VLLRSSKKGA EGSACAPGEA KAAVQCSFCK
181SQFERKKDLE LHVHQAHKPF KCRLCSYATL REESLLSHIE RDHITAQGPG SGEACVENGK
241PELSPGEFPC EVCGQAFSQT WFLKAHMKKH RGSFDHGCHI CGRRFKEPWF LKNHMKAHGP
301KTGSKNRPKS ELDPIATINN VVQEEVIVAG LSLYEVCAKC GNLFTNLDSL NAHNAIHRRV
361EASRTRAPAE EGAEGPSDTK QFFLQCLNLR PSAAGDSCPG TQAGRRVAEL DPVNSYQAWQ
421LATRGKVAEP AEYLKYGAWD EALAGDVAFD KDRREYVLVS QEKRKREQDA PAAQGPPRKR
481ASGPGDPAPA GHLDPRSAAR PNRRAAATTG QGKSSECFEC GKIFRTYHQM VLHSRVHRRA
541RRERDSDGDR AARARCGSLS EGDSASQPSS PGSACAAADS PGSGLADEAA EDSGEEGAPE
601PAPGGQPRRC CFSEEVTSTE LSSGDQSHKM GDNASERDTG ESKAGIAASV SILENSSRET
661SRRQEQHRFS MDLKMPAFHP KQEVPVPGDG VEFPSSTGAE GQTGHPAEKL SDLHNKEHSG
721GGKRALAPDL MPLDLSARST RDDPSNKETA SSLQAALVVH PCPYCSHKTY YPEVLWMHKR
781IWHRVSCNSV APPWIQPNGY KSIRSNLVFL SRSGRTGPPP ALGGKECQPL LLARFTRTQV
841PGGMPGSKSG SSPLGVVTKA ASMPKNKESH SGGPCALWAP GPDGYRQTKP CHGQEPHGAA
901TQGPLAKPRQ EASSKPVPAP GGGGFSRSAT PTPTVIARAG AQPSANSKPV EKFGVPPAGA
961GFAPTNKHSA PDSLKAKFSA QPQGPPPAKG EGGAPPLPPR EPPSKAAQEL RTLATCAAGS
1021RGDAALQAQP GVAGAPPVLH SIKQEPVAEG HEKRLDILNI FKTYIPKDFA TLYQGWGVSG
1081PGLEHRGTLR TQARPGEFVC IECGKSFHQP GHLRAHMRAH SVVFESDGPR GSEVHTTSAD
1141APKQGRDHSN TGTVQTVPLR KGT
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1163 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.