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ZNF513 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF513 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G106972-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF513 (NM_144631), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HMFT0656 | RP58 | ZNF513 | Zinc finger protein 513
Origin Species
Human
SwissProt
Q8N8E2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MPRRKQSHPQ PVKCEGVKVD TEDSLDEGPG ALVLESDLLL GQDLEFEEEE EEEEGDGNSD
61QLMGFERDSE GDSLGARPGL PYGLSDDESG GGRALSAESE VEEPARGPGE ARGERPGPAC
121QLCGGPTGEG PCCGAGGPGG GPLLPPRLLY SCRLCTFVSH YSSHLKRHMQ THSGEKPFRC
181GRCPYASAQL VNLTRHTRTH TGEKPYRCPH CPFACSSLGN LRRHQRTHAG PPTPPCPTCG
241FRCCTPRPAR PPSPTEQEGA VPRRPEDALL LPDLSLHVPP GGASFLPDCG QLRGEGEGLC
301GTGSEPLPEL LFPWTCRGCG QELEEGEGSR LGAAMCGRCM RGEAGGGASG GPQGPSDKGF
361ACSLCPFATH YPNHLARHMK THSGEKPFRC ARCPYASAHL DNLKRHQRVH TGEKPYKCPL
421CPYACGNLAN LKRHGRIHSG DKPFRCSLCN YSCNQSMNLK RHMLRHTGEK PFRCATCAYT
481TGHWDNYKRH QKVHGHGGAG GPGLSASEGW APPHSPPSVL SSRGPPALGT AGSRAVHTDS
541S
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

541 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.