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ZNF512B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF512B Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G111027-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF512B (NM_020713), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GM632 | Zinc finger protein 512B | ZNF512B | KIAA1196
Origin Species
Human
NCBI GenBank
AB033022
SwissProt
Q96KM6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
97.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTDPFCVGGR RLPGSSKSGP GKDGSRKEVR LPMLHDPPKM GMPVVRGGQT VPGQAPLCFD
61PGSPASDKTE GKKKGRPKAE NQALRDIPLS LMNDWKDEFK AHSRVKCPNS GCWLEFPSIY
121GLKYHYQRCQ GGAISDRLAF PCPFCEAAFT SKTQLEKHRI WNHMDRPLPA SKPGPISRPV
181TISRPVGVSK PIGVSKPVTI GKPVGVSKPI GISKPVSVGR PMPVTKAIPV TRPVPVTKPV
241TVSRPMPVTK AMPVTKPITV TKSVPVTKPV PVTKPITVTK LVTVTKPVPV TKPVTVSRPI
301VVSKPVTVSR PIAISRHTPP CKMVLLTRSE NKAPRATGRN SGKKRAADSL DTCPIPPKQA
361RPENGEYGPS SMGQSSAFQL SADTSSGSLS PGSRPSGGME ALKAAGPASP PEEDPERTKH
421RRKQKTPKKF TGEQPSISGT FGLKGLVKAE DKARVHRSKK QEGPGPEDAR KKVPAAPITV
481SKEAPAPVAH PAPGGPEEQW QRAIHERGEA VCPTCNVVTR KTLVGLKKHM EVCQKLQDAL
541KCQHCRKQFK SKAGLNYHTM AEHSAKPSDA EASEGGEQEE RERLRKVLKQ MGRLRCPQEG
601CGAAFSSLMG YQYHQRRCGK PPCEVDSPSF PCTHCGKTYR SKAGHDYHVR SEHTAPPPEE
661PTDKSPEAED PLGVERTPSG RVRRTSAQVA VFHLQEIAED ELARDWTKRR MKDDLVPETA
721RLNYTRPGLP TLNPQLLEAW KNEVKEKGHV NCPNDCCEAI YSSVSGLKAH LASCSKGAHL
781AGKYRCLLCP KEFSSESGVK YHILKTHAEN WFRTSADPPP KHRSQDSLVP KKEKKKNLAG
841GKKRGRKPKE RTPEEPVAKL PPRRDDWPPG CRDKGARGST GRKVGVSKAP EK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

892 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.