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ZNF512 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF512 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G108822-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF512 (NM_032434), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 512 | ZNF512 | KIAA1805
Origin Species
Human
NCBI GenBank
NM_032434
SwissProt
Q96ME7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSSRLGAVPA TSGPTTFKQQ RSTRIVGAKN SRTQCSIKDN SFQYTIPHDD SLSGSSSASS
61CEPVSDFPAS FRKSTYWMKM RRIKPAATSH VEGSGGVSAK GKRKPRQEED EDYREFPQKK
121HKLYGRKQRP KTQPNPKSQA RRIRKEPPVY AAGSLEEQWY LEIVDKGSVS CPTCQAVGRK
181TIEGLKKHME NCKQEMFTCH HCGKQLRSLA GMKYHVMANH NSLPILKAGD EIDEPSERER
241LRTVLKRLGK LRCMRESCSS SFTSIMGYLY HVRKCGKGAA ELEKMTLKCH HCGKPYRSKA
301GLAYHLRSEH GPISFFPESG QPECLKEMNL ESKSGGRVQR RSAKIAVYHL QELASAELAK
361EWPKRKVLQD LVPDDRKLKY TRPGLPTFSQ EVLHKWKTDI KKYHRIQCPN QGCEAVYSSV
421SGLKAHLGSC TLGNFVAGKY KCLLCQKEFV SESGVKYHIN SVHAEDWFVV NPTTTKSFEK
481LMKIKQRQQE EEKRRQQHRS RRSLRRRQQP GIELPETELS LRVGKDQRRN NEELVVSASC
541KEPEQEPVPA QFQKVKPPKT NHKRGRK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

567 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.