Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
ZNF509 / ZBTB49 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G106728-100
Catalog Number: LS-G106728
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF509(ZBTB49) (NM_145291), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ZBTB49 | Zinc finger protein 509 | ZNF509
- Origin Species
- Human
- SwissProt
- Q6ZSB9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 84.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MDPVATHSCH LLQQLHEQRI QGLLCDCMLV VKGVCFKAHK NVLAAFSQYF RSLFQNSSSQ61KNDVFHLDVK NVSGIGQILD FMYTSHLDLN QDNIQVMLDT AQCLQVQNVL SLCHTFLKSA121TVVQPPGMPC NSTLSLQSTL TPDATCVISE NYPPHLLQEC SADAQQNKTL DESHPHASPS181VNRHHSAGEI SKQAPDTSDG SCTELPFKQP NYYYKLRNFY SKQYHKHAAG PSQERVVEQP241FAFSTSTDLT TVESQPCAVS HSECILESPE HLPSNFLAQP VNDSAPHPES DATCQQPVKQ301MRLKKAIHLK KLNFLKSQKY AEQVSEPKSD DGLTKRLESA SKNTLEKASS QSAEEKESEE361VVSCENFNCI SETERPEDPA ALEDQSQTLQ SQRQYACELC GKPFKHPSNL ELHKRSHTGE421KPFECNICGK HFSQAGNLQT HLRRHSGEKP YICEICGKRF AASGDVQRHI IIHSGEKPHL481CDICGRGFSN FSNLKEHKKT HTADKVFTCD ECGKSFNMQR KLVKHRIRHT GERPYSCSAC541GKCFGGSGDL RRHVRAHTGE KPYTCEICNK CFTRSAVLRR HKKMHCKAGD ESPDVLEELS601QAIETSDLEK SQSSDSFSQD TSVTLMPVSV KLPVHPVENS VAEFDSHSGG SYCKLRSMIQ661PHGVSDQEKL SLDPGKLAKP QMQQTQPQAY AYSDVDTPAG GEPLQADGMA MIRSSLAALD721NHGGDPLGSR ASSTTYRNSE GQFFSSMTLW GLAMKTLQNE NELDQSGPSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
768 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.