LSBio
ZNF500 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF500 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G110621-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF500 (NM_021646), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0557 | Zinc finger protein 500 | ZKSCAN18 | ZSCAN50 | ZNF500
Origin Species
Human
NCBI GenBank
NM_021646
SwissProt
O60304

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.5 kda
Protein Type
Lysate
Amino Acid Sequence
1MATVPGLQPL PTLEQDLEQE EILIVKVEED FCLEEEPSVE TEDPSPETFR QLFRLFCYQE
61VAGPREALSR LWELCCRWLR PELRTKEQIL ELLVLEQFLT VLPGEIQARV REQQPESGEE
121AVVLVEGLQR KPRKHRQRGS ELLSDDEVPL GIGGQFLKHQ AEAQPEDLSL EEEARFSSQQ
181PPAQLSHRPQ RGPLLWPERG PPAPRHQEMA SASPFLSAWS QVPVNLEDVA VYLSGEEPRC
241MDPAQRDAPL ENEGPGIQLE DGGDGREDAP LRMEWYRVLS ARCQGPGHPL PGQRPAPVRG
301LVRPDQPRGG PPPGRRASHG ADKPYTCPEC GKGFSKTSHL TKHQRTHTGE RPYKCLVCGK
361GFSDRSNFST HQRVHTGEKP YPCPECGKRF SQSSSLVIHR RTHSGERPYA CTQCGKRFNN
421SSHFSAHRRT HTGEKPYTCP ACGRGFRRGT DLHKHQRTHM GAGSLPTLQP VAPGGPGAKA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

480 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.