LSBio
ZNF498 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF498 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ZSCAN25 antibodies →
$725.00each
Catalog Number: LS-G106736-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF498(ZSCAN25) (NM_145115), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ZNF498 | Zinc finger protein 498 | ZSCAN25
Origin Species
Human
NCBI GenBank
NM_145115
SwissProt
Q6NSZ9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLKEHPEMAE APQQQLGIPV VKLEKELPWG RGREDPSPET FRLRFRQFRY QEAAGPQEAL
61RELQELCRRW LRPELHTKEQ ILELLVLEQF LTILPREFYA WIREHGPESG KALAAMVEDL
121TERALEAKAV PCHRQGEQEE TALCRGAWEP GIQLGPVEVK PEWGMPPGEG VQGPDPGTEE
181QLSQDPGDET RAFQEQALPV LQAGPGLPAV NPRDQEMAAG FFTAGSQGLG PFKDMALAFP
241EEEWRHVTPA QIDCFGEYVE PQDCRVSPGG GSKEKEAKPP QEDLKGALVA LTSERFGEAS
301LQGPGLGRVC EQEPGGPAGS APGLPPPQHG AIPLPDEVKT HSSFWKPFQC PECGKGFSRS
361SNLVRHQRTH EEKSYGCVEC GKGFTLREYL MKHQRTHLGK RPYVCSECWK TFSQRHHLEV
421HQRSHTGEKP YKCGDCWKSF SRRQHLQVHR RTHTGEKPYT CECGKSFSRN ANLAVHRRAH
481TGEKPYGCQV CGKRFSKGER LVRHQRIHTG EKPYHCPACG RSFNQRSILN RHQKTQHRQE
541PLVQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

544 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.