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ZNF497 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF497 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G103706-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF497 (NM_198458), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Zinc finger protein 497 | Zinc finger-like protein | ZNF497
Origin Species
Human
NCBI GenBank
NM_198458
SwissProt
Q6ZNH5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MESPRGWTLQ VAPEEGQVLC NVKTATRGLS EGAVSGGWGA WENSTEVPRE AGDGQRQQAT
61LGAADEQGGP GRELGPADGG RDGAGPRSEP ADRALRPSPL PEEPGCRCGE CGKAFSQGSY
121LLQHRRVHTG EKPYTCPECG KAFAWSSNLS QHQRIHSGEK PYACRECGKA FRAHSQLIHH
181QETHSGLKPF RCPDCGKSFG RSTTLVQHRR THTGEKPYEC PECGKAFSWN SNFLEHRRVH
241TGARPHACRD CGKAFSQSSN LAEHLKIHAG ARPHACPDCG KAFVRVAGLR QHRRTHSSEK
301PFPCAECGKA FRESSQLLQH QRTHTGERPF ECAECGQAFV MGSYLAEHRR VHTGEKPHAC
361AQCGKAFSQR SNLLSHRRTH SGAKPFACAD CGKAFRGSSG LAHHRLSHTG ERPFACAECG
421KAFRGSSELR QHQRLHSGER PFVCAHCSKA FVRKSELLSH RRTHTGERPY ACGECGKPFS
481HRCNLNEHQK RHGGRAAP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

498 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.