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ZNF490 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ZNF490 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111030-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZNF490 (NM_020714), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1198 | Zinc finger protein 490 | ZNF490
Origin Species
Human
SwissProt
Q9ULM2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRRNSSLSFQ MERPLEEQVQ SKWSSSQGRT GTGGSDVLQM QNSEHHGQSI KTQTDSISLE
61DVAVNFTLEE WALLDPGQRN IYRDVMRATF KNLACIGEKW KDQDIEDEHK NQGRNLRSPM
121VEALCENKED CPCGKSTSQI PDLNTNLETP TGLKPCDCSV CGEVFMHQVS LNRHMRSHTE
181QKPNECHEYG EKPHKCKECG KTFTRSSSIR THERIHTGEK PYECKECGKA FAFLFSFRNH
241IRIHTGETPY ECKECGKAFR YLTALRRHEK NHTGEKPYKC KQCGKAFIYY QPFLTHERTH
301TGEKPYECKQ CGKAFSCPTY LRSHEKTHTG EKPFVCRECG RAFFSHSSLR KHVKTHTGVQ
361PYTCKKCGEA FKSSSSCEVH ERTHFGEKPY ECKQCGKAFN SSSYLQLHER VHTGEKTYEC
421KECGKAFLYS THFRIHERTH TREKPYECKQ CGRVFIYFSH LRRHERSHTG VKPCECKQCG
481KAFTCLNSLK VHKRIHTGER PFQCRQCGKA FSYSKSLHVH ERTHSRQKP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

529 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.